Zaman, UzmaUzmaZamanUrlaub, HenningHenningUrlaubAbbasi, AtiyaAtiyaAbbasi2023-10-062023-10-062017https://resolver.sub.uni-goettingen.de/purl?gro-2/134323Abstract Introduction Cumin ( Cuminum cyminum ) , a popular spice has been widely used in traditional medicine to cure various ailments. Despite the existence of scientific literature about its pharmacological properties, no successful proteome profiling has yet been attempted. Objective To optimise extraction of cumin proteins and analyse its profile by shotgun proteomics, using one‐dimensional electrophoresis coupled with nano‐ESI‐LC–MS/MS. Methodology As a first step, we have compared three extraction protocols for total proteins extraction from cumin. Extracted proteins were separated on one‐dimensional gel and analysed by state‐of‐the‐art linear ion trap (LTQ)‐Orbitrap Velose and Q Exactive HF mass spectrometer. Results Evaluation of extraction method revealed significant differences in protein yield and proteome composition between the three extracts. LC–MS/MS allowed identification of several proteins with functional significance in various biological processes. Conclusion This study provides identification of a large number of proteins and offers a molecular basis for future research on potential pharmacologically active cumin proteins. Copyright © 2017 John Wiley & Sons, Ltd.Cuminum cyminum (cumin) is a popular spice and traditional medicine. The present study was designed for in depth proteome profiling of cumin by shotgun proteomics, using one dimensional electrophoresis coupled with nano‐ESI‐LC‐MS/MS as no successful data has yet been reported. In addition, evaluation of three protein extraction protocols revealed significant difference in protein yield and proteome composition. Identification of high number of functionally significant proteins provides a molecular basis for future research on potential pharmacologically active proteins of cumin.enhttp://onlinelibrary.wiley.com/termsAndConditions#vorjournal_article10.1002/pca.2738