Stahl, MarkusMarkusStahlSchuh, ReinhardReinhardSchuhAdryan, BorisBorisAdryan2018-11-072018-11-072007https://resolver.sub.uni-goettingen.de/purl?gro-2/52352The embryonic development of the tracheal system of the fruit fly Drosophila provides a paradigm for genetic studies of branching morphogenesis. Efforts of many laboratories have identified Branchless (Bnl, a fibroblast growth factor homologue) and Breathless (Btl, the receptor homologue) as crucial factors at many stages of tracheal system development. The downstream targets of the Bnl/Btl signalling cascade, however, remain mostly unknown. Misexpression of the bnl gene results in specific tracheal phenotypes that lead to larval death. We characterised the transcriptional profiles of targeted over-expression of bnl in the embryonic trachea and of loss-of-function bnl(PI) mutant embryos. Gene expression data was mapped to high-throughput in situ hybridisation based ImaGO-annotation. Thus, we identified and confirmed by quantitative PCR 13 Bnl-dependent genes that are expressed in cells within and outside of the tracheal system. (c) 2006 Elsevier B.V. All rights reserved.journal_article10.1016/j.modgep.2006.07.00516949886000242739100026