Naumann, Peter-ThomasPeter-ThomasNaumannLauhon, Charles T.Charles T.LauhonFicner, RalfRalfFicner2017-09-072017-09-072013https://resolver.sub.uni-goettingen.de/purl?gro-2/7486The sulfurtransferase 4-thiouridine synthetase (ThiI) is involved in the ATP-dependent modification of U8 in tRNA. ThiI from Thermotoga maritima was cloned, overexpressed and purified. A complex comprising ThiI and a truncated tRNA was prepared and crystallized, and X-ray diffraction data were collected to a resolution of 3.5 angstrom. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 102.9, b = 112.8, c = 132.8 angstrom.journal_article10.1107/S1744309113004077235456500003169491000163142366