Quintanilla-Licea, RamiroRamiroQuintanilla-LiceaMorado-Castillo, RolandoRolandoMorado-CastilloGomez-Flores, RicardoRicardoGomez-FloresLaatsch, HartmutHartmutLaatschJulia Verde-Star, MariaMariaJulia Verde-StarHernandez-Martinez, HumbertoHumbertoHernandez-MartinezTamez-Guerra, PatriciaPatriciaTamez-GuerraTamez-Guerra, ReyesReyesTamez-GuerraRodriguez-Padilla, CristinaCristinaRodriguez-Padilla2018-11-072018-11-072012https://resolver.sub.uni-goettingen.de/purl?gro-2/25528Bioassay-guided fractionation of hexane extracts of Gymnosperma glutinosum (Asteraceae) leaves, collected in North Mexico, afforded the known compounds hentriacontane (1) and (+)-13S, 14R, 15-trihydroxy-ent-labd-7-ene (2), as well as the new ent-labdane diterpene (-)-13S, 14R, 15-trihydroxy-7-oxo-ent-labd-8(9)-ene (3). In addition, D-glycero-D-galactoheptitol (4) was isolated from the methanolic extract of this plant. Their structures were established on the basis of high-field 1D- and 2D NMR methods supported by HR-MS data. The cytotoxic activity was determined by using the in vitro L5178Y-R lymphoma murine model. Hentriacontane (1) and the new ent-labdane 3 showed weak cytotoxicity, whereas the ent-labdane 2 showed significant (p < 0.05) and concentration dependent cytotoxicity (up to 78%) against L5178Y-R cells at concentrations ranging from 7.8 to 250 mu g/mL.journal_article10.3390/molecules17091122922996346000309269700087