Sajid, ImranImranSajidShaaban, Khaled AttiaKhaled AttiaShaabanHasnain, ShahidaShahidaHasnain2018-11-072018-11-072011https://resolver.sub.uni-goettingen.de/purl?gro-2/24508A new actinomycete strain designated as Streptomyces sp. CTF15 was isolated from a saline soil using casein-KNO3 agar medium. The strain Streptomyces sp. CTF15 exhibited promising antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Streptomyces viridochromogens Tu57 and high cytotoxicity (91.2% mortality) against Artimia salina in biological screening. The cultivation of this strain in a 50 L lab fermenter and subsequent isolation and purification by a series of chromatographic techniques and structure elucidation by MS and NMR analysis of the active metabolites revealed that it is a highly stable producer of resistomycin (1), tetracenomycin D (2) and actinomycin D (3), even under non-optimised culture conditions. The morphological, microscopic, biochemical and physiological characterisation suggested that the strain CTF15 belongs to the genus Streptomyces. A partial 16S rRNA gene sequence (1429 bp) from the strain CTF15 was determined and found to have high identity (99%) with Streptomyces griseoincarnatus. As such, this is the first report of a strain of S. griseoincarnatus capable of producing these three bioactive compounds simultaneously.journal_article10.1080/14786419.2010.53499321391117000288270400006