Tug-of-War and Coordination in Bidirectional Transport by Molecular Motors

Abstract

Many cargoes in cells are transported in a bidirectional fashion by molecular motors pulling into opposite directions along a cytoskeletal filament, e.g., by kinesins and dyneins along microtubules. How opposite-polarity motors are coordinated has been under debate for a long time, with experimental evidence supporting both a tug-of-war between the motors as well as biochemical coordination mechanisms. Here we propose a model that extends a tug-of-war model by a mechanism of motor activation and inactivation and show that this model can explain some observations that are incompatible with a simple tug-of-war scenario, specifically long unidirectional runs and a directional memory after unbinding from the filament. Both features are present in two variants of the model in which motors are activated and inactivated individually and in opposite-direction pairs, respectively.