Two new chiral EDTA-based metal chelates for weak alignment of proteins in solution

Abstract

A short synthesis of EDTA-based metal chelates that can be attached to the cysteine residue of a protein via a disuffide bond is described. The complexes were used after coordination of lanthanides to align trigger factor and apo-calmodulin in solution to yield residual dipolar couplings and pseudocontact shifts. Alignment tensors for the new tags are linearly independent compared to those of previously published tags.