Differential modulation of parietal cortex activity by respiration and θ oscillations

Abstract

Nasal respiration generates a rhythmic signal that entrains large portions of the mammalian brain into respiration-coupled field potentials. Here, we report the simultaneous presence of respiratory rhythm (RR) and θ oscillations in the parietal association cortex of mice. Despite their overlapping frequencies, both rhythms differ in their state-dependent power and differentially entrain the discharge behavior of units. We conclude that network activity in the parietal cortex is synchronized by two different physiological oscillation patterns.

The simultaneous, local integration of information from widespread brain regions is an essential feature of cortical computation and particularly relevant for multimodal association areas such as the posterior parietal cortex. Slow, rhythmic fluctuations in the local field potentials (LFPs) are assumed to constitute a global signal aiding interregional communication through the long-range synchronization of neuronal activity. Recent work demonstrated the brain-wide presence of a novel class of slow neuronal oscillations that are entrained by nasal respiration. However, whether there are differences in the influence of the respiration-entrained rhythm (RR) and the endogenous theta (θ) rhythm over local networks is unknown. In this work, we aimed at characterizing the impact of both classes of oscillations on neuronal activity in the posterior parietal cortex of mice. We focused our investigations on a θ-dominated state (rapid eye movement sleep) and an RR-dominated state (wake immobility). Using linear silicon probes implanted along the dorsoventral cortical axis, we found that the LFP-depth distributions of both rhythms show differences in amplitude and coherence but no phase shift. Using tetrode recordings, we demonstrate that a substantial fraction of parietal neurons is modulated by either RR or θ or even by both rhythms simultaneously. Interestingly, the phase and cortical depth dependence of spike-field coupling differ for these oscillations. We further show through intracellular recordings in urethane-anesthetized mice that synaptic inhibition is likely to play a role in generating respiration-entrainment at the membrane potential level. We conclude that θ and respiration differentially affect neuronal activity in the parietal cortex. NEW & NOTEWORTHY Nasal respiration generates a rhythmic signal that entrains large portions of the mammalian brain into respiration-coupled field potentials. Here, we report the simultaneous presence of respiratory rhythm (RR) and θ oscillations in the parietal association cortex of mice. Despite their overlapping frequencies, both rhythms differ in their state-dependent power and differentially entrain the discharge behavior of units. We conclude that network activity in the parietal cortex is synchronized by two different physiological oscillation patterns.