Diversification and Taxonomy of the Liverwort Jubula Dumort. (Jungermanniopsida: Porellales) Inferred from Nuclear and Chloroplast DNA Sequences

Abstract

Nucleatide sequence variation in the ITSI-5.8S-ITS2 region Of nuclear ribosomal DNA and in the trnL-trnF region of cpDNA from 28 specimens of Jabula (Jubulaceae), and two outgroup species, was analyzed using maximum parsimony and maximum likelihood. The molecular topologies supported five to six allopatric taxa in Jubula. Relationships of most of these taxa remain unresolved due to lack of support of many deeper nodes. A principal component analysis indicated a broad overlap of morphological characters of these taxa. Based on the morphological similarities of the Jubula taxa and the observed low sequence variation, a subspecies concept is supported. This comprises the taxa J. hutchinsiae subsp. bogotensis, subsp. hutchinsiae, subsp. japonica, subsp. javanica, and subsp. pennsylvanica. With the exception of J. hutchinsiae subsp, javanica, all taxa are nionophyletic. Multiple accessions of the temperate taxa J. hutchinsiae subsp. hutchinsiae and J. hutchinsiae subsp. pennsylvanica exhibit a lower sequence variation than the tropical taxa J. hutchinsiac subsp. begotensis and J. hutchinsine subsp. javanica