Browsing by Author "Scheich, Henning"

The effects of early onset, unilateral conductive hearing loss on tone-induced 2-deoxyglucose (2-DG) uptake in the auditory cortex of juvenile Mongolian gerbils (Meriones unguiculatus) were studied. Atresia of the left ear canal was induced at postnatal day 9 (P9) to achieve reversible auditory deprivation prior to onset of hearing (around P12). Atresia either persisted (ATR, n = 4) or the canal was opened 15 min before the 2-DG experiments (RE, n = 4) at P27. Control animals were either non-deprived (CON, n = 4), or their left ears were plugged acutely (PAX, it = 4). In PAX, 2-DG uptake in primary auditory cortex (AI) and anterior auditory field (AAF) was lower in right than in left AI and AAF. In contrast, in ATR and RE, uptake was significantly higher on the right side contralateral to the atresia. Hence, atresia during early development leads to plastic changes resulting in an interhemispheric imbalance of functional metabolism in favor of the auditory cortex contralateral to the manipulated ear. Distances between tone-induced 2-DG labeling in AI and AAF were increased in PAX, but smaller in ATR in the right compared to the left hemisphere, suggesting effects of atresia also on spatial relations in cortical tonotopic maps. (C) 2000 Elsevier Science B.V. All rights reserved.

In neurons the rate of K(+)-uptake increases with increasing activity. K(+)-analogues like the heavy metal ion thallium (Tl(+)) can be used, therefore, as tracers for imaging neuronal activity. However, when water-soluble Tl(+)-salts are injected systemically only minute amounts of the tracer enter the brain and the Tl(+)-uptake patterns are influenced by regional differences in blood-brain barrier (BBB) K(+)-permeability. We here show that the BBB-related limitations in using Tl(+) for imaging neuronal activity are no longer present when the lipophilic Tl(+) chelate complex thallium diethyldithiocarbamate (TlDDC) is applied. We systemically injected rodents with TlDDC and mapped the Tl(+)-distribution in the brain using an autometallographic (AMG) technique, a histochemical method for detecting heavy metals. We find that Tl(+)-doses for optimum AMG staining Could be substantially reduced, and regional differences attributable to differences in BBB K(+)-permeability were no longer detectable, indicating that TlDDC crosses the BBB. At the cellular level, however, the Tl(+)-distribution was essentially the same as after injection of water-soluble Tl(+)-salts, indicating Tl(+)-release from TlDDC prior to neuronal or glial uptake. Upon sensory stimulation or intracortical microstimulation neuronal Tl(+)-uptake increased after TlDDC injection, upon muscimol treatment neuronal Tl(+)-uptake decreased. We present a protocol for mapping neuronal activity with cellular resolution, which is based oil intravenous TlDDC injections during ongoing activity in unrestrained behaving animals and short stimulation times of 5 min. (C) 2009 Elsevier Inc. All rights reserved.