Browsing by Author "Leiser, R."

Binucleate trophoblast giant cells (BNC) in the bovine placenta produce glycoproteins, which are delivered into the mother after fusion of BNC with uterine epithelial cells. During most time of pregnancy, BNC produce pregnancy-associated glycoproteins (PAGs) and prolactin-related protein-I (PRP-1) with asparagine-linked lactosamine-type glycans terminating with N-acetyl-galactosamine. We show by lectin histochernistry that terminal N-acetyl-galactosamine (detected by Dolichos biflorus agglutinin, DBA) in placentornal BNC is greatly reduced prior to parturition, while lactosamine-type N-glycans (detected by Phaseolus vulgaris leucoagglutinin, PHA-L) remain unaltered. The change in DBA-staining showed no statistically significant differences between placentomes of cows with and without retention of fetal membranes. Western blots revealed that, at parturition the apparent molecular mass of PAGs and PRP-I is 1-2 kDa lower than in late pregnancy. These changes are due to alterations of asparagine-linked glycans, since the molecular weight of the peptide backbones after enzymatical release of asparagine-linked glycans is identical at late pregnancy and parturition. Lectin western blots showed a reduction of terminal N-acetyl-galactosamine on PAGs at parturition. A lectin sandwich-E LISA was used to differentiate DBA- and PHA-L-binding PAGs in sera of pregnant and non-pregnant cows. The values for DBA-binding PACs at parturition were not significantly different from non-pregnancy, while the values for PHA-L-binding PACs were significantly higher at parturition. The peripartal changes of PAG- and PRP-1-glycosylation could alter functional properties of these proteins and might therefore be considered for functional studies. The differentiation of PAC glycoforms in maternal serum could be valuable for a further optimization of PAG-based pregnancy diagnosis in cattle.